Across TCGA pan-cancer cohorts, H3-3A mass-spec protein differs between tumor and matched normal tissue in 2 of 18 cancer types tested, making tumor–normal expression one of H3-3A’s most consistent transcriptional readouts.
The strongest signal is observed in clear cell renal cell carcinoma (CCRCC), where H3-3A mass-spec protein is more highly expressed in tumor relative to normal tissue. In most cancer types H3-3A is over-expressed in tumor.
CCRCC and LUAD are the cancer types where H3-3A tumor–normal differential expression is most reproducible.