Across TCGA pan-cancer cohorts, AFDN-DT RNA differs between tumor and matched normal tissue in 12 of 18 cancer types tested, making tumor–normal expression one of AFDN-DT’s most consistent transcriptional readouts.
The strongest signal is observed in head and neck squamous cell carcinoma (HNSC), where AFDN-DT RNA is repressed in tumor relative to normal tissue. In most cancer types AFDN-DT is over-expressed in tumor, although a few such as HNSC and KICH show the opposite, repressed pattern.
HNSC, KICH, and COAD are the cancer types where AFDN-DT tumor–normal differential expression is most reproducible.
RNA tumor vs normal associations by lineage
Ranked by sampling consensus. Fold-change is the tumor-versus-normal difference in AFDN-DT RNA (log2); positive values indicate higher expression in tumor. p-values are from the differential-expression test.