Across TCGA pan-cancer cohorts, H2AX mass-spec protein differs between tumor and matched normal tissue in 6 of 18 cancer types tested, making tumor–normal expression one of H2AX’s most consistent transcriptional readouts.
The strongest signal is observed in colon adenocarcinoma (COAD), where H2AX mass-spec protein is more highly expressed in tumor relative to normal tissue. In most cancer types H2AX is over-expressed in tumor, although a few such as PDAC show the opposite, repressed pattern.
COAD, CCRCC, and PDAC are the cancer types where H2AX tumor–normal differential expression is most reproducible.
mass-spec protein tumor vs normal associations by lineage
Ranked by sampling consensus. Fold-change is the tumor-versus-normal difference in H2AX mass-spec protein (log2); positive values indicate higher expression in tumor. p-values are from the differential-expression test.