Across TCGA pan-cancer cohorts, DHFR mass-spec protein is linked to patient survival in 5 of 34 cancer types, making it a survival-associated DHFR data layer compared with 24 for mass-spec protein and 1 for mutation status.

The strongest signal is observed in lung adenocarcinoma (LUAD), where higher DHFR mass-spec protein is associated with worse overall survival. In most high-consensus cancer types, elevated DHFR expression acts as an unfavorable survival marker, although some lineages such as HNSC and LSCC show a favorable association.

LUAD, CCRCC, and HNSC are the cancer types where DHFR mass-spec protein most reproducibly stratifies survival.

mass-spec protein survival associations by lineage

Ranked by sampling consensus. AUC1 and AUC2 indicate survival in the high- and low-expression groups, respectively; the lower AUC marks the poorer-surviving group. p-values are from the log-rank test.

Lineage	Measure	Split	Stage	AUC1 high	AUC2 low	p	Sampling consensus
LUAD	OS	Tertile	All	0.459	1.000	<.001	31	view →
CCRCC	OS	Median	II,III,IV	0.327	0.896	.037	3	view →
HNSC	DFS	Median	IV	0.889	0.407	.042	2	view →
PDAC	OS	Median	All	0.809	0.896	.048	1	view →
LSCC	DFS	Quartile	All	1.000	0.892	.043	1	view →

Pink = unfavorable, green = favorable. Showing the 5 strongest of 5 lineages.

Exploration

Same gene, other analysis: DHFR tumor-vs-normal RNA expression · all DHFR data types